Mouse Prostaglandin D2(PGD2) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14022m

Sensitivity

2.5 pg/mL

Detection Range

5 pg/mL-1000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Prostaglandin D2(PGD2)

Recovery

The recovery of human AXL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-99
EDTA plasma (n=4)	95	87-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AXL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	84-95
1:2	Average %	92
	Range %	85-99
1:4	Average %	90
	Range %	86-95
1:8	Average %	95
	Range %	85-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	PGD2

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Activated niacin receptor HCA2 inhibits chemoattractant-mediated macrophage migration via GBr/PKC/ERK1/2 pathway and heterologous receptor desensitization Ying Shi,.et al,Scientific Reports,7: 42279.Feb 10. G2A Protects Mice against Sepsis by Modulating Kupffer Cell Activation: Cooperativity with Adenosine Receptor 2b Li HM, et al,Journal of immunology,December 10

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