Mouse Progesterone,PROG ELISA Kit

Mouse Progesterone,PROG ELISA Kit

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Mfr.No.

CSB-E05104m

Alternative Names

Progesterone,PROG

Reactivity

Mouse

Detection Range

0.3 ng/mL-10 ng/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	85-98
EDTA plasma (n=4)	94	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	83
	Range %	81-99
1:2	Average %	95
	Range %	90-105
1:4	Average %	85
	Range %	80-91
1:8	Average %	92
	Range %	80-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
80	2.567	2.502	2.535	2.393
40	1.851	1.816	1.834	1.692
20	1.301	1.243	1.272	1.130
10	0.852	0.809	0.831	0.689
5	0.532	0.514	0.523	0.381
2.5	0.383	0.376	0.380	0.238
1.25	0.247	0.239	0.243	0.101
0	0.145	0.139	0.142	?

Species

Mus musculus

Sensitivity

0.2 ng/mL

Limit Of Detection

450 nm

Antigen	progesterone,PROG

Applications

ELISA

Research Area

PROG

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Anthrax Toxin Receptor 2 Functions in ECM Homeostasis of the Murine Reproductive Tract and Promotes MMP Activity Reeves CV et al ,PLOS ONE,2012 7(4):e34862 Superovulation Induced Changes of Lipid Metabolism in Ovaries and Embryos and Its Probable Mechanism Wang LY. et al,PLoS One,2015 Jul 13;10(7):e0132638. Bisphenol A Initiates Excessive Premature Activation of Primordial Follicles in Mouse Ovaries via the PTEN Signaling Pathway Hu Y.et al,Reprod Sci.,Oct 5 Silica nanoparticle exposure inducing granulosa cell apoptosis and follicular atresia in female Balb/c mice Liu J.et al,Environ Sci Pollut Res Int,Nov 19

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SKU	Supplier No.	Size	Your price
BHE10506487	CSB-E05104m	96T	$495	Add to Cart
BHE10506487	CSB-E05104m	5x96T	$2376	Add to Cart
BHE10506487	CSB-E05104m	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.