Mouse procollagen type III (HPCIII) ELISA Kit

Mfr.No.

CSB-E17125m

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

procollagen type III (HPCIII), procollagen type 3 ,HPC3, procollagen type III ,HPCIII procollagen type III ,HPCIII, procollagen type III (HPCIII), procollagen type 3 ,HPC3, procollagen type III ,HPCIII procollagen type III ,HPCIII

Recovery

The recovery of pig TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	110	103-118

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	97
	Range %	92-103
1:4	Average %	85
	Range %	81-87
1:8	Average %	89
	Range %	83-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.539	2.672	2.606	2.500
10	1.855	1.988	1.922	1.816
5	0.881	0.885	0.883	0.777
2.5	0.387	0.411	0.399	0.293
1.25	0.249	0.255	0.252	0.146
0.625	0.168	0.178	0.173	0.067
0.312	0.140	0.141	0.141	0.035
0	0.103	0.108	0.106	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	HPCIII

Storage	Store at 2-8°C. Please refer to protocol.

Reference	SIRT3 Facilitates Amniotic Fluid Stem Cells to Repair Diabetic Nephropathy Through Protecting Mitochondrial Homeostasis by Modulation of Mitophagy Feng J.et al,Cellular Physiology and Biochemistry,April 19

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SKU	Supplier No.	Size	Your price
BHE10506485	CSB-E17125m	96T	$595	Add to Cart
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BHE10506485	CSB-E17125m	10x96T	$5652.50	Add to Cart

Mouse procollagen type III (HPCIII) ELISA Kit

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