Mouse Pro-Nerve growth factor (proNGF) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027721MO

Sensitivity

5.8 pg/mL

Detection Range

23.5 pg/mL-1500 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Alternative Names

Pro-Nerve growth factor (proNGF)

Recovery

The recovery of rat GDF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-90
EDTA plasma (n=4)	93	89-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat GDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:10	Average %	92
	Range %	87-96
1:20	Average %	83
	Range %	80-86
1:40	Average %	96
	Range %	93-99
1:80	Average %	102
	Range %	99-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	proNGF

Storage	Store at 2-8°C. Please refer to protocol.

Reference	RIP2 Gates TRAF6 Interaction with Death Receptor p75NTR to Regulate Cerebellar Granule Neuron Survival LilianKisiswa.et al,Cell reports,July 24

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