Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of PR3-ANCA and the recovery rates were calculated by comparing the measured value to the expected amount of PR3-ANCA in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
94
EDTA plasma(n=5)
85-101
91
heparin plasma(n=5)
86-100
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PR3-ANCA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
96-105%
89-99%
85-97%
EDTA plasma(n=5)
88-100%
83-94%
90-96%
heparin plasma(n=5)
81-88%
85-92%
80-98%
Sensitivity
< 37.5pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
PR3-ANCAELISA Kit allows for the in vitro quantitative determination of PR3-ANCA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse PR3-ANCA (proteinase-? anti-neutrophil cytoplasmic antibody) ELISA Kit