Mouse Pou Domain - Class 5 - Transcription Factor 1

Mfr.No.

E2357Mo

Sensitivity

9.52ng/L

Detection Range

20-6000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

44472;44473;NF-A3;Oct;Oct-;Oct3/;Oct-3/;Oct3/4;Oct-3/4;Octamer-binding protein 3;Octamer-binding protein 4;Octamer-binding transcription factor 3;Otf;Otf-;Otf3-;Otf-3;Otf3g;Otf3-rs7;Otf4;Otf-4;POU domain, class 5, transcription factor 1;POU5F1;

Molecular Weight

38216

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2357Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	869.4	43.09	4.96
Intra-Assay	2	18	1535.42	56.91	3.71
Intra-Assay	3	18	584.33	35.06	6

Background

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') (PubMed:1972777, PubMed:1690859, PubMed:1967980, PubMed:17525163, PubMed:23376973). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (PubMed:15863505, PubMed:17097055, PubMed:17496161, PubMed:19740739). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:1972777, PubMed:1690859, PubMed:17496161, PubMed:18662995, PubMed:19740739, PubMed:29153991, PubMed:23376973).

Protocol

These standard curves of E2357Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.366	2.268	2.228
	2.171
1600ng/L	1.373	1.386	1.345
	1.398
800ng/L	0.701	0.7	0.66
	0.699
400ng/L	0.469	0.429	0.388
	0.388
200ng/L	0.19	0.228	0.188
	0.267
0ng/L	0.047	0.04	0
	0.033

Gene ID	18999
UniProt ID	P20263

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110168	E2357Mo	48T	$320		Add to Cart
BHE12110168	E2357Mo	96T	$458		Add to Cart

Mouse Pou Domain - Class 5 - Transcription Factor 1 - POU5F1 ELISA Kit

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