Mouse Plexin A1/PLXNA1 ELISA Kit PicoKine®

Mfr.No.

EK1818

Sensitivity

<0.1 ng/ml

Detection Range

0.312 ng/ml - 20 ng/ml

Reactivity

Mouse

Sample Volume

Concentration (ng/ml)	0	0.3	0.63	1.25	2.5	5	10	20
O.D.	0.158	0.232	0.286	0.326	0.607	0.935	1.409	2.343

Description

Mouse Plexin A1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Plxna1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Plexin-A1; Plex 1; Plexin-1; Plxna1; Kiaa4053

Immunogen

Expression system for standard: NS0; Immunogen sequence: S28-P1242

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Plexin A1

Subcellular Localization

Cell membrane, Single-pass type I membrane protein

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	462	2424	8962	500	2463	9301
Standard deviation	21.71	123.62	412.25	27.00	155.17	697.58
CV (%)	4.7%	5.1%	4.6%	5.4%	6.3%	7.5%

Protein Function

Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex compos

Background

Plexin-A1 is a protein that in humans is encoded by the PLXNA1 gene. This gene is mapped to 6; 6 D1. Plexin A1 has been shown to interact with AKT1.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	462	417	452	485	454	24.48	5.3%
Sample 2	2424	2827	2492	2766	2627	172.30	6.5%
Sample 3	8962	10130	10185	9716	9748	488.80	5.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	18844
Gene Name	Plxna1
UniProt ID	P70206
Tissue Specificity	Ubiquitous.

Applications	ELISA
Research Area	Axonal Guidance Proteins,Growth and Development,More Gpcr,Neurogenesis,Neurology Process,Neuroscience,Neurotransmission,Receptors / Channels

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001419	EK1818	96 wells/kit	$750		Add to Cart

Mouse Plexin A1/PLXNA1 ELISA Kit PicoKine®

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