Mouse Platelet-Derived Growth Factor Soluble Receptor alpha,PDGFsR-alpha ELISA kit

Mouse Platelet-Derived Growth Factor Soluble Receptor alpha,PDGFsR-alpha ELISA kit

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Mfr.No.

CSB-E04699m

Alternative Names

platelet-derived growth factor receptor, alpha polypeptide, CD140A, MGC74795, PDGFR2, RHEPDGFRA, CD140 antigen-like family member A, PDGF-R-alpha, PDGFRA/BCR fusion, platelet-derived growth factor receptor alpha, rearranged-in-hypereosinophilia-platelet derived

Reactivity

Mouse

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human APOC4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	98	94-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human APOC4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	92
	Range %	88-96
1:400	Average %	87
	Range %	84-90
1:800	Average %	95
	Range %	91-99
1:1600	Average %	97
	Range %	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
400	0.080	0.083	0.082
200	0.117	0.119	0.118
100	0.177	0.172	0.175
50	0.276	0.296	0.286
25	0.504	0.490	0.497
12.5	0.923	0.931	0.927
6.25	1.461	1.496	1.479
0	2.539	2.587	2.563

Species

Mus musculus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	P26618
Antigen	platelet-derived growth factor receptor, alpha polypeptide

Applications

ELISA

Research Area

PDGFRA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506461	CSB-E04699m	96T	$835	Add to Cart
BHE10506461	CSB-E04699m	5x96T	$4008	Add to Cart
BHE10506461	CSB-E04699m	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.