Mouse platelet activating factor,PAF ELISA KIT

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08199m

Sensitivity

0.195 ng/mL

Detection Range

0.78 ng/mL-50 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

platelet activating factor,PAF

Recovery

The recovery of human apo-C3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	97	90-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human apo-C3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	87-99
1:200	Average %	89
	Range %	81-94
1:400	Average %	101
	Range %	91-105
1:800	Average %	100
	Range %	90-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	PAF

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Cigarette smoke promotes COPD by activating platelet-activating factor receptor and inducing neutrophil autophagic death in mice Lv XX.et al,Oncotarget,Sep 26 Pneumolysin mediates heterotypic aggregation of neutrophils and platelets in vitro Nel JG.et al,J Infect,Mar 5 Mast cells contribute to Enterovirus 71 infection-induced pulmonary edema in neonatal mice Yuefei Jin.et al,Laboratory Investigation ,May 15

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