Mouse Plastin-2(LCP1) ELISA kit

Mouse Plastin-2(LCP1) ELISA kit

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Mfr.No.

CSB-EL012824MO

Sensitivity

6.25 pg/mL

Detection Range

25 pg/mL-1600 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

lymphocyte cytosolic protein 1 (L-plastin), CP64, DKFZp781A23186, FLJ25423, FLJ26114, FLJ39956, L-PLASTIN, LC64P, LPL, PLS2, L-plastin, L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P), Lymphocyte cytosolic protein-1 (plasmin), OTTHUMP

Recovery

The recovery of horse LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	86-100
EDTA plasma (n=4)	104	100-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of horse LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	99
	Range %	95-104
1:200	Average %	103
	Range %	98-109
1:400	Average %	89
	Range %	84-95
1:800	Average %	96
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
200	0.054	0.056	0.055
100	0.094	0.099	0.097
50	0.181	0.183	0.182
25	0.310	0.302	0.306
12.5	0.594	0.546	0.570
6.25	0.995	0.985	0.990
3.12	1.330	1.346	1.338
0	1.684	1.605	1.645

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q61233

Applications	ELISA
Research Area	LCP1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506454	CSB-EL012824MO	96T	$695	Add to Cart
BHE10506454	CSB-EL012824MO	5x96T	$3336	Add to Cart
BHE10506454	CSB-EL012824MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.