Mouse plasminogen activator inhibitor 1,PAI-1 ELISA Kit

Mfr.No.

CSB-E07947m

Sensitivity

0.22 ng/mL

Detection Range

0.9 ng/mL-60 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, PAI, PAI-1, PAI1, PLANH1, plasminogen activator inhibitor-1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

Recovery

The recovery of human APOL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	104	98-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human APOL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	98
	Range %	92-106
1:40	Average %	93
	Range %	88-97
1:80	Average %	104
	Range %	102-107
1:160	Average %	88
	Range %	84-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
500	0.145	0.147	0.146
250	0.241	0.253	0.247
125	0.383	0.397	0.390
62.5	0.601	0.613	0.607
31.25	0.857	0.862	0.860
15.62	1.112	1.155	1.134
7.81	1.597	1.643	1.620
0	1.981	1.999	1.990

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P22777

Applications	ELISA
Research Area	SERPINE1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506451	CSB-E07947m	96T	$835	Add to Cart
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BHE10506451	CSB-E07947m	10x96T	$7932.50	Add to Cart

Mouse plasminogen activator inhibitor 1,PAI-1 ELISA Kit

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