Mouse plasmin-antiplasmin complex,PAP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08436m

Sensitivity

0.78 pg/mL

Detection Range

3.12 pg/mL-200 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

plasmin-antiplasmin complex,PAP

Recovery

The recovery of rat IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-95
EDTA plasma (n=4)	98	94-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-94
1:2	Average %	104
	Range %	100-107
1:4	Average %	92
	Range %	87-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	PAP

Storage	Store at 2-8°C. Please refer to protocol.

Reference	The role of plasmin in the pathogenesis of murine multiple myeloma Eiamboonsert S.et al,Biochem Biophys Res Commun.,May 10 Plasminogen activator inhibitor-1 regulates macrophage-dependent postoperative adhesion by enhancing EGF-HER1 signaling in mice Kumpei Honjo.et al,FASEB J.,Mar 7 The fibrinolytic factor tPA drives LRP1-mediated melanoma growth and metastasis Salama Y, et al,Faseb journal,November 20

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