Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine®

Mfr.No.

EK2107

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.023	0.151	0.288	0.492	0.811	1.242	1.611	2.006

Description

Mouse KLKB1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Klkb1 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Plasma kallikrein; Fletcher factor; Kininogenin; Plasma prekallikrein; PKK; Plasma kallikrein heavy chain; Plasma kallikrein light chain; KLKB1; KLK3

Immunogen

Expression system for standard: NS0; Immunogen sequence: G20-A638

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse KLKB1

Subcellular Localization

Secreted

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	247	1236	4806	295	1245	4921
Standard deviation	11.12	70.45	221.08	20.95	85.91	314.94
CV (%)	4.5%	5.7%	4.6%	7.1%	6.9%	6.4%

Protein Function

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

Background

Plasma kallikrein?is a?protein?that in humans is encoded by the?KLKB1?gene. It is mapped to 4q35.2. This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alteative splicing results in multiple transcript variants encoding different isoforms.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	252	275	235	272	258	16.19	6.2%
Sample 2	1856	1776	1691	1826	1787	62.48	3.4%
Sample 3	4296	4210	3832	4617	4238	279.56	6.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	16621
Gene Name	Klkb1
UniProt ID	P26262

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001655	EK2107	96 wells/kit	$750		Add to Cart

Mouse Plasma Kallikrein/KLKB1 ELISA Kit PicoKine®

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