Matrices listed below were spiked with certain level of Pla2g2a and the recovery rates were calculated by comparing the measured value to the expected amount of Pla2g2a in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-103
99
EDTA plasma(n=5)
91-101
97
heparin plasma(n=5)
89-100
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pla2g2a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-96%
85-102%
85-99%
EDTA plasma(n=5)
89-100%
91-101%
93-101%
heparin plasma(n=5)
93-100%
94-99%
91-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P31482
Applications
ELISA
Applications Notes
Pla2g2a ELISA Kit allows for the in vitro quantitative determination of Pla2g2a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Pla2g2a(Phospholipase A2, membrane associated) ELISA Kit