Mouse PLA2G2A(Phospholipase A2, Group IIA) ELISA Kit

Mfr.No.

ELK6949

Sensitivity

6.5 pg/mL

Detection Range

15.63-1000 pg/mL

Reactivity

Mouse

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

PLA2; MOM1; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2(platelets,synovial fluid); Non-pancreatic secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A

Recovery

Matrices listed below were spiked with certain level of recombinant PLA2G2A and the recovery rates were calculated by comparing the measured value to the expected amount of PLA2G2A in samples.

Matrix	Recovery range	Average
serum(n=5)	88-102%	95%
EDTA plasma(n=5)	83-96%	89%
Heparin plasma(n=5)	92-107%	99%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PLA2G2A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	93-104%	83-96%	95-103%	88-95%
EDTA plasma(n=5)	88-97%	91-103%	86-92%	89-97%
Heparin plasma(n=5)	85-94%	87-96%	89-101%	82-90%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.115	2.027
500.00	1.502	1.414
250.00	1.174	1.086
125.00	0.726	0.638
62.50	0.587	0.499
31.25	0.239	0.151
15.63	0.124	0.036
0.00	0.088	0.000

Limit Of Detection

1000 pg/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PLA2G2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PLA2G2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PLA2G2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PLA2G2A in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

UniProt ID	P31482

Applications	ELISA
Research Area	Enzyme & Kinase;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15206272	ELK6949	48T	$320		Add to Cart
BHE15206272	ELK6949	96T	$458		Add to Cart

Mouse PLA2G2A(Phospholipase A2, Group IIA) ELISA Kit

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