Matrices listed below were spiked with certain level of PIIICP and the recovery rates were calculated by comparing the measured value to the expected amount of PIIICP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-101
95
EDTA plasma(n=5)
86-97
92
heparin plasma(n=5)
88-103
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PIIICP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
PIIICP ELISA Kit allows for the in vitro quantitative determination of PIIICP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse PIIICP(c-terminal Procollagen III propeptide) ELISA Kit