Mouse Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) ELISA kit

Mouse Phospholipid hydroperoxide glutathione peroxidase, mitochondrial(GPX4) ELISA kit

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Mfr.No.

CSB-EL009869MO

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

glutathione peroxidase 4 (phospholipid hydroperoxidase), MCSP, PHGPx, snGPx, snPHGPx, glutathione peroxidase 4, phospholipid hydroperoxidase, phospholipid hydroperoxide glutathione peroxidase, mitochondrial, sperm nucleus glutathione peroxidase

Recovery

The recovery of human METRNL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-103
EDTA plasma (n=4)	92	88-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human METRNL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-89
1:2	Average %	90
	Range %	86-96
1:4	Average %	95
	Range %	90-99
1:8	Average %	93
	Range %	86-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.695	2.587	2.641	2.471
5	2.079	2.136	2.108	1.938
2.5	1.682	1.604	1.643	1.473
1.25	1.266	1.169	1.218	1.048
0.625	0.719	0.694	0.707	0.537
0.312	0.399	0.427	0.413	0.243
0.156	0.218	0.209	0.214	0.044
0	0.173	0.167	0.170	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q91XR9

Applications	ELISA
Research Area	GPX4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506438	CSB-EL009869MO	96T	$695	Add to Cart
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BHE10506438	CSB-EL009869MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.