Mouse Phosphoglycerate Mutase 1

Mfr.No.

E2342Mo

Alternative Names

BPG-dependent PGAM 1;PGAM 1;PGAM1;PGAM-B;Phosphoglycerate mutase 1;Phosphoglycerate mutase isozyme B

Reactivity

Mouse

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

28832

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.65ng/L

Background

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.

Gene ID	18648
UniProt ID	Q9DBJ1
Antigen	PGAM1

Research Area

Signal Transduction

Protocol

These standard curves of E2342Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.103	2.21	2.151
	2.317
800ng/L	1.384	1.373	1.314
	1.362
400ng/L	0.818	0.784	0.724
	0.749
200ng/L	0.505	0.463	0.404
	0.421
100ng/L	0.308	0.312	0.252
	0.315
0ng/L	0.068	0.059	0
	0.05

Precision

We measured random samples of E2342Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	965.2	28.52	3
Intra-Assay	2	18	639.6	34.54	5.4
Intra-Assay	3	18	300.5	18.31	6.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110153	E2342Mo	48T	$320		Add to Cart
BHE12110153	E2342Mo	96T	$458		Add to Cart

Mouse Phosphoglycerate Mutase 1 - PGAM1 ELISA Kit

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