Mouse Phosphoglycerate Kinase 1

Mfr.No.

E2341Mo

Sensitivity

9.04ng/L

Detection Range

20-6000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

PGK 1;PGK1;Phosphoglycerate kinase 1

Molecular Weight

44550

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2341Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	864.4	48.92	5.7
Intra-Assay	2	18	2121.6	90.55	4.3
Intra-Assay	3	18	318.3	18.38	5.8

Background

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). May play a role in sperm motility.

Protocol

These standard curves of E2341Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.137	2.118	2.069
	2.098
1600ng/L	1.342	1.404	1.355
	1.466
800ng/L	0.952	0.897	0.848
	0.842
400ng/L	0.5	0.483	0.434
	0.465
200ng/L	0.31	0.284	0.235
	0.258
0ng/L	0.044	0.049	0
	0.054

Gene ID	18655
UniProt ID	P09411

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110152	E2341Mo	48T	$320		Add to Cart
BHE12110152	E2341Mo	96T	$458		Add to Cart

Mouse Phosphoglycerate Kinase 1 - PGK1 ELISA Kit

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