Mouse Persephin ELISA Kit PicoKine®

Mfr.No.

EK1499

Sensitivity

<10 pg/ml

Detection Range

1.56 pg/ml - 100 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.017	0.071	0.136	0.226	0.533	0.978	1.533	2.053

Description

Mouse Persephin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Pspn in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Persephin;PSP;Pspn;

Molecular Weight

17031 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: W22-G156

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Persephin

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	3.7	12	49	3.6	11.4	55
Standard deviation	0.18	0.94	2.3	0.23	1.026	2.86
CV (%)	4.9%	7.8%	4.7%	6.5%	9%	5.2%

Protein Function

Exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons.

Background

Persephin, also known as PSPN, is a neurotrophic factor in the glial cell line-derived neurotrophic factor (GDNF) family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease. This PSPN gene is mapped to chromosome 19p13.3. The encoded protein has amino acid similarity to its other family members, glial cell line-derived neurotrophic factor and neurturin. This gene product promotes the survival of ventral midbrain dopaminergic neurons in culture and prevents their degeneration after 6-hydroxydopamine treatment in vivo.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	3726	3810	3.849	3.865	3.812	53.79	1.4%
Sample 2	12.058	12.135	11.622	10.357	11.543	712.13	6.1%
Sample 3	49.927	48.196	52.754	50.855	50.433	1645.09	3.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	19197
Gene Name	Pspn
UniProt ID	O70300
Protein Name	Persephin

Applications	ELISA
Research Area	Growth and Development,Neurology Process,Neuroscience

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001098	EK1499	96 wells/kit	$750		Add to Cart

Mouse Persephin ELISA Kit PicoKine®

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