Mouse Peroxisome Proliferator-activated receptor gamma,PPAR-gamma ELISA Kit

Mouse Peroxisome Proliferator-activated receptor gamma,PPAR-gamma ELISA Kit

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Mfr.No.

CSB-E08625m

Sensitivity

0.156 pg/mL

Detection Range

0.625 pg/mL-40 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

peroxisome proliferator-activated receptor gamma, CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARgamma, OTTHUMP00000185030, OTTHUMP00000185033, PPAR gamma, nuclear receptor subfamily 1 group C member 3, peroxisome proliferative activated receptor gamma, peroxi

Recovery

The recovery of rat Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	94	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	92
	Range %	87-98
1:200	Average %	98
	Range %	93-104
1:400	Average %	84
	Range %	82-88
1:800	Average %	90
	Range %	85-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
1000	0.067	0.065	0.066
500	0.121	0.125	0.123
250	0.213	0.204	0.209
125	0.386	0.355	0.371
62.5	0.604	0.590	0.597
31.2	0.811	0.858	0.835
15.6	1.109	1.116	1.113
0	2.155	2.214	2.185

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P37238

Applications	ELISA
Research Area	PPARG

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10506426	CSB-E08625m	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.