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Home / ELISA Kit

Mouse Perforin/Pore-forming protein (PF/PFP) ELISA Kit

SKU : BHE13705843

Pore-forming proteins (Also known as Pore-forming toxins) are protein exotoxins, usually produced by bacteria, such as C.septicum and S.aureus. They are frequently cytotoxic (i.e., they kill cells), as they create unregulated pores in the membrane of targeted cells

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Mfr.No.	KTE70684
Description	This Mouse Perforin/Pore-forming protein (PF/PFP) ELISA Kit employs a two-site sandwich ELISA to quantitate PRF1.
Alternative Names	PRF1; RP11-710A11.3; FLH2; HPLH2; MGC65093; P1; PFN1; PFP; OTTHUMP00000019759; cytolysin; lymphocyte pore forming protein; perforin 1
Reactivity	Mouse
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Mouse Perforin/Pore-forming protein microplate Mouse Perforin/Pore-forming protein standard Mouse Perforin/Pore-forming protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Perforin/Pore-forming protein (PF/PFP) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRF1. No significant cross-reactivity or interference between Mouse PRF1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Pore-forming proteins (Also known as Pore-forming toxins) are protein exotoxins, usually produced by bacteria, such as C.septicum and S.aureus. They are frequently cytotoxic (i.e., they kill cells), as they create unregulated pores in the membrane of targeted cells
Notes	This Mouse Perforin/Pore-forming protein (PF/PFP) ELISA Kit employs a two-site sandwich ELISA to quantitate PRF1 in samples. An antibody specific for PRF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	18646
Antigen	Prf1
Tag	PRF1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Mouse Perforin/Pore-forming protein (PF/PFP) ELISA Kit employs a two-site sandwich ELISA to quantitate PRF1 in samples. An antibody specific for PRF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705843	KTE70684	48 T	$359		Add to Cart
BHE13705843	KTE70684	96 T	$619		Add to Cart
BHE13705843	KTE70684	96 Tx5	$2809		Add to Cart
BHE13705843	KTE70684	96 Tx50	$26209		Add to Cart

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