Mouse PDGF-BB ELISA Kit PicoKine®

Mfr.No.

EK1697

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.066	0.045	0.129	0.198	0.478	0.991	1.817	2.424

Description

Mouse PDGF-BB ELISA Kit PicoKine® (96 Tests). Quantitate Mouse PDGF-BB in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Epidermal growth factor-like protein 7; EGF-like protein 7; Multiple epidermal growth factor-like domains protein 7; Multiple EGF-like domains protein 7; NOTCH4-like protein; Vascular endothelial statin; VE-statin; Zneu1; Egfl7; Megf7

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S82-T190

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse PDGF-BB

Subcellular Localization

Secreted, extracellular space

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	149	683	1203	150	750	1331
Standard deviation	6.7	48.49	56.54	7.95	43.5	82.5
CV (%)	4.5%	7.1%	4.7%	5.3%	5.8%	6.2%

Protein Function

Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cell adhesion to the extracellular matrix and angiogenesis.

Background

PDGF-BB encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alteatively spliced transcript variants encoding different isoforms have been identified for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	149	172	178	169	167	10.88	6.5%
Sample . 2	683	696	665	663	676	13.57	2.0%%
Sample 3	1203	1369	1343	1427	1335	82.32	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	18591
Gene Name	PDGF-BB
UniProt ID	P31240
Tissue Specificity	Expressed specifically by endothelial cells of the highly vascularized organs heart, lung and kidney.

Applications	ELISA
Research Area	Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Growth Factors,Growth Factors/Hormones,Immunology,Metabolism,Metabolism Processes,Neurology Process,Neuroscience,Pathways and Processes,Platelets,Response To Hypoxia,Secreted Molecules,Serum Proteins,Signal Transduction,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001363	EK1697	96 wells/kit	$499		Add to Cart

Mouse PDGF-BB ELISA Kit PicoKine®

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