Mouse Pde5a(cGMP-specific 3',5'-cyclic phosphodiesterase) ELISA Kit

Mfr.No.

EM0388

Alternative Names

Pde5a ELISA Kit, cGMP-binding cGMP-specific phosphodiesterase ELISA Kit, CGB-PDE ELISA Kit, PDE5 ELISA Kit

Reactivity

Mouse

Detection Method

Double Antibody

Detection Range

78.125-5000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Pde5a and the recovery rates were calculated by comparing the measured value to the expected amount of Pde5a in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	87-104	97
EDTA plasma(n=5)	88-97	92
heparin plasma(n=5)	90-103	97

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pde5a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-105%	91-102%	86-105%
EDTA plasma(n=5)	84-92%	88-101%	83-100%
heparin plasma(n=5)	80-91%	84-100%	90-99%

Sensitivity

< 46.875pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

Pde5a/cGMP-binding cGMP-specific phosphodiesterase(CGB-PDE)/PDE5

Notes

For Research Use Only

UniProt ID	Q8CG03
Antigen	PDE5A

Applications	ELISA
Applications Notes	Pde5a ELISA Kit allows for the in vitro quantitative determination of Pde5a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10801633	EM0388	96T	$400		Add to Cart

Mouse Pde5a(cGMP-specific 3 ,5 -cyclic phosphodiesterase) ELISA Kit

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