Matrices listed below were spiked with certain level of Pck1 and the recovery rates were calculated by comparing the measured value to the expected amount of Pck1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-97
90
EDTA plasma(n=5)
85-97
92
heparin plasma(n=5)
92-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pck1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Pck1 ELISA Kit allows for the in vitro quantitative determination of Pck1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Pck1(Phosphoenolpyruvate carboxykinase, cytosolic [GTP]) ELISA Kit