Mouse Pancreatic Triacylglycerol Lipase

Mfr.No.

E1413Mo

Sensitivity

0.14ng/ml

Detection Range

0.3-90ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Mouse Nesfatin1 (also known as PL/PNLIP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Pancreatic lipase;Pancreatic triacylglycerol lipase;PL;PNLIP;PTL

Molecular Weight

51428

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1413Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	32.7	1.23	3.8
Intra-Assay	2	18	16.5	0.73	4.4
Intra-Assay	3	18	6.5	0.25	3.8

Background

Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.

Protocol

These standard curves of E1413Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.303	2.181	2.134
	2.059
24ng/ml	1.471	1.436	1.389
	1.401
12ng/ml	0.627	0.699	0.653
	0.772
6ng/ml	0.318	0.326	0.279
	0.333
3ng/ml	0.196	0.193	0.147
	0.191
0ng/ml	0.046	0.047	0
	0.047

Gene ID	69060
UniProt ID	Q6P8U6

Research Area	Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109276	E1413Mo	48T	$320		Add to Cart
BHE12109276	E1413Mo	96T	$458		Add to Cart

Mouse Pancreatic Triacylglycerol Lipase - PNLIP ELISA Kit

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