Mouse Palmitoyl-protein Thioesterase 1

Mfr.No.

E2325Mo

Alternative Names

Palmitoyl-protein hydrolase 1;Palmitoyl-protein thioesterase 1;PPT 1;PPT1;PPT-1

Reactivity

Mouse

Detection Range

20-3500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

34490

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.62ng/L

Background

Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or Peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.

Gene ID	19063
UniProt ID	O88531
Antigen	PPT1

Research Area

Others

Protocol

These standard curves of E2325Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.62	2.558	2.499
	2.495
1000ng/L	1.511	1.465	1.407
	1.419
500ng/L	0.713	0.788	0.73
	0.863
250ng/L	0.498	0.468	0.409
	0.437
125ng/L	0.225	0.245	0.186
	0.264
0ng/L	0.053	0.058	0
	0.064

Precision

We measured random samples of E2325Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	978.1	31.86	3.3
Intra-Assay	2	18	844.2	42.43	5
Intra-Assay	3	18	277.6	12.71	4.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110136	E2325Mo	48T	$320		Add to Cart
BHE12110136	E2325Mo	96T	$458		Add to Cart

Mouse Palmitoyl-protein Thioesterase 1 - PPT1 ELISA Kit

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