Mouse P-Selectin / CD62P ELISA Kit PicoKine®

Mfr.No.

EK0506

Sensitivity

<5 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	312	625	1250	2500	5000	10000	20000
O.D.	0.013	0.144	0.227	0.388	0.593	1.073	1.616	2.198

Description

Mouse P-Selectin / CD62P ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Selp in cell culture supeatants, serum and plasma (citrate). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

P-selectin;CD62 antigen-like family member P;Granule membrane protein 140;GMP-140;Leukocyte-endothelial cell adhesion molecule 3;LECAM3;Platelet activation dependent granule-exteal membrane protein;PADGEM;CD62P;Selp;Grmp;

Molecular Weight

83099 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: W42-A709

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse sP-Selectin

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	596	2016	7521	640	1834	7467
Standard deviation	28.6	106.84	443.73	32	105%	500.28
CV (%)	4.8%	5.3%	5.9%	5%	5.9%	6.7%

Protein Function

Ca (2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. .

Background

P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1). The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	596	594	610	551	587	22.09	3.7%
Sample 2	2016	2063	1888	1979	1986	64.18	3.2%
Sample 3	7521	7765	7893	8387	7891	315.75	4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	20344
Gene Name	SELP
UniProt ID	Q01102
Protein Name	P-selectin
Tissue Specificity	Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Sequence	Belongs to the selectin/LECAM family.

Applications	ELISA
Research Area	Adaptive Immunity,Angiogenesis,Atherosclerosis,Cardiovascular,Cell Adhesion Molecules,Cell Type Markers,Hematopoietic Progenitors,Immunology,Myeloid,Platelets,Stem Cells,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000486	EK0506	96 wells/kit	$499		Add to Cart

Mouse P-Selectin / CD62P ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse P-Selectin / CD62P ELISA Kit PicoKine®

Rate it now..!!