Mouse oxidized low density lipoprotein,OxLDL ELISA Kit

Mouse oxidized low density lipoprotein,OxLDL ELISA Kit

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Mfr.No.

CSB-E07933m

Sensitivity

0.11 nmol/mL

Detection Range

0.45 nmol/mL-30 nmol/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

oxidized lowdensity lipoprotein,OxLDL

Recovery

The recovery of rabbit Apo-B100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-102
EDTA plasma (n=4)	87	82-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Apo-B100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	90-102
1:200	Average %	92
	Range %	87-96
1:400	Average %	104
	Range %	98-108
1:800	Average %	85
	Range %	80-89

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
5000	0.092	0.091	0.092
2500	0.146	0.138	0.142
1250	0.246	0.236	0.241
625	0.375	0.360	0.368
312	0.524	0.539	0.532
156	0.934	0.877	0.906
78	1.447	1.334	1.391
0	2.432	2.562	2.497

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	OxLDL

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Increased Blood-Brain Barrier Permeability and Brain Edema After Focal Cerebral Ischemia Induced by Hyperlipidemia Ayman ElAli et al,Stroke,2011 Nov;42(11):3238-44. Lactobacillus acidophilus ATCC 4356 attenuates the atherosclerotic progression through modulation of oxidative stress and inflammatory process Chen L et al,Int Immunopharmacol,2013 Jun 5.pii: S1567-5769(13)00216-6. Periodontal Pathogen Accelerates Lipid Peroxidation and Atherosclerosis Jia R et al,J Dent Res,2013 Mar;92(3):247-52. Oxidatively modified LDL particles in the human placenta in early and late onset intrauterine growth restriction Ulrich Pecks et al,Placenta,Available online 16October 2013 Sublingual Vaccine with GroEL Attenuates Atherosclerosis Hagiwara M et al,J Dent Res,2014 Feb 19. Effects of flavonol-rich green tea cultivar (Camellia sinensis L.) on plasma oxidized LDL levels in hypercholesterolemic mice Nomura S. et al,Biosci Biotechnol Biochem,Sep 12:1-3. P2X7R is involved in the progression of atherosclerosis by promoting NLRP3 inflammasome activation Peng K et al,Int J Mol Med,2015 Mar 9 Inhibition of Glutathione Production Induces Macrophage CD36 Expression and Enhances Cellular-oxidized Low Density Lipoprotein (oxLDL) Uptake. Yang X.et al,J Biol Chem.,290(36):21788-99 Orange beverage ameliorates high-fat-diet-induced metabolic disorder in mice Escudero-Lpez B.et al,Journal of Functional Foods,24: 254-263 Low ambient oxygen prevents atherosclerosis. Kang JG. et al,J Mol Med (Berl).,2016 Feb 2. Proatherosclerotic Effect of the a1-Subunit of Soluble Guanylyl Cyclase by Promoting Smooth Muscle Phenotypic Switching. Segura-Puimedon M.et al,Am J Pathol.,S0002-9440(16)30124-9 ox-LDL induces endothelial dysfunction by promoting Arp2/3 complex expression Tang Y.et al,Biochem Biophys Res Commun.,475(2):182-8 Sex-dependent co-occurrence of hypoxia and β-amyloid plaques in hippocampus and entorhinal cortex is reversed by long-term treatment with ubiquinol and ascorbic acid in the 3 × Tg-AD mouse model of Alzheimer's disease Javier.et al,Molecular and Cellular Neuroscience,June 25

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.