Matrices listed below were spiked with certain level of OVA sIgG2b and the recovery rates were calculated by comparing the measured value to the expected amount of OVA sIgG2b in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
82-104
96
EDTA plasma(n=5)
83-105
96
heparin plasma(n=5)
86-98
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OVA sIgG2b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
80-105%
83-99%
85-105%
EDTA plasma(n=5)
90-100%
90-100%
90-100%
heparin plasma(n=5)
91-97%
91-95%
94-98%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
OVA sIgG2b ELISA Kit allows for the in vitro quantitative determination of OVA sIgG2b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse OVA sIgG2b (Ovalbumin Specific IgG2b) ELISA Kit