Matrices listed below were spiked with certain level of OVA sIgG2a and the recovery rates were calculated by comparing the measured value to the expected amount of OVA sIgG2a in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-104
98
EDTA plasma(n=5)
85-102
92
heparin plasma(n=5)
81-105
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OVA sIgG2a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-102%
86-96%
84-105%
EDTA plasma(n=5)
95-100%
92-101%
93-99%
heparin plasma(n=5)
91-98%
90-97%
93-99%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
OVA sIgG2a ELISA Kit allows for the in vitro quantitative determination of OVA sIgG2a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse OVA sIgG2a (Ovalbumin Specific IgG2a) ELISA Kit