Mouse OSCAR ELISA Kit PicoKine®

Mfr.No.

EK2281

Sensitivity

<10 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration(pg/ml)	0	78	156	312	625	1,250	2,500	5,000
O.D.	0.055	0.106	0.158	0.334	0.699	1.098	1.379	1.869

Description

Mouse OSCAR ELISA Kit PicoKine® (96 Tests). Quantitate Mouse OSCAR in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D19-N228

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human LY96

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	85	218	2107	108	167	2056
Standard deviation	2.11	7.56	107.04	4.27	4.63	134.67
CV(%)	2.5%	3.5%	5.1%	4%	2.8%	6.6%

Background

Osteoclasts are multinucleated cells that resorb bone and are essential for bone homeostasis. This gene encodes an osteoclast-associated receptor (OSCAR), which is a member of the leukocyte receptor complex protein family that plays critical roles in the regulation of both innate and adaptive immune responses. The encoded protein may play a role in oxidative stress-mediated atherogenesis as well as monocyte adhesion. Multiple alteatively spliced transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	85	104	86	140	104	4.59	4.4%
Sample 2	218	289	184	163	214	14.43	6.8%
Sample 3	2107	2156	1988	1334	1896	41.53	2.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	232790
Gene Name	Oscar
UniProt ID	Q8VBT3

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001830	EK2281	96 wells/kit	$750		Add to Cart

Mouse OSCAR ELISA Kit PicoKine®

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