Mouse Orexin A ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08861m

Alternative Names

Orexin A

Reactivity

Mouse

Detection Range

78 pg/mL-5000 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse APOB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	105	102-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	102
	Range %	97-108
1:200	Average %	103
	Range %	97-106
1:400	Average %	92
	Range %	88-97
1:800	Average %	86
	Range %	82-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

19.5 pg/mL

Limit Of Detection

450 nm

UniProt ID	O55241
Antigen	Orexin A

Applications

ELISA

Research Area

Orexin A

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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