Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine®

Mfr.No.

EK0481

Sensitivity

<5 pg/ml

Detection Range

93.7 pg/ml - 6,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.000	0.082	0.173	0.294	0.522	0.976	1.585	2.018

Description

Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tnfrsf11b in cell culture supeatants, serum and plasma (EDTA). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Tumor necrosis factor receptor superfamily member 11B;Osteoclastogenesis inhibitory factor;Osteoprotegerin;Tnfrsf11b;Ocif, Opg;

Molecular Weight

45923 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E22-L401

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse OPG

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	133	800	3535	139	767	3643
Standard deviation	5.85	54.4	187.35	7.39	55.39	225.86
CV (%)	4.4%	6.8%	5.3%	4.6%	7.3%	6.2%

Protein Function

Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. .

Background

Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	133	157	157	146	148	9.88	6.6%
Sample 2	800	766	743	710	754	32.84	4.3%
Sample 3	3535	3949	3581	3584	3662	166.69	4.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	18383
Gene Name	TNFRSF11B
UniProt ID	O08712
Protein Name	Tumor necrosis factor receptor superfamily member 11B
Tissue Specificity	Highly expressed in liver, lung, stomach, intestines and calvaria. Highly expressed in decidua and placenta, and in embryo.
Sequence	Contains 2 death domains.

Applications	ELISA
Research Area	Atherosclerosis,Cardiovascular,Cytokines,Cytoskeleton/ECM,Extracellular Matrix,Immunology,Innate Immunity,Metabolism,Obesity,Signal Transduction,Structures,TNF Superfamily,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000471	EK0481	96 wells/kit	$499		Add to Cart

Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine®

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