Mouse nuclear factor-kappaB p65,NF-kappaB p65 ELISA Kit

Mfr.No.

CSB-E08789m

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

v-rel reticuloendotheliosis viral oncogene homolog A (avian), MGC131774, NFKB3, p65, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene

Recovery

The recovery of mouse STC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	99	95-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse STC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-97
1:2	Average %	97
	Range %	95-102
1:4	Average %	101
	Range %	95-105
1:8	Average %	94
	Range %	91-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.558	2.633	2.596	2.387
2000	2.205	2.212	2.209	2.000
1000	1.682	1.665	1.674	1.465
500	0.990	0.995	0.993	0.784
250	0.499	0.512	0.506	0.297
125	0.390	0.421	0.406	0.197
62.5	0.299	0.322	0.311	0.102
0	0.205	0.212	0.209	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q04207

Applications	ELISA
Research Area	RELA

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506373	CSB-E08789m	96T	$595	Add to Cart
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BHE10506373	CSB-E08789m	10x96T	$5652.50	Add to Cart

Mouse nuclear factor-kappaB p65,NF-kappaB p65 ELISA Kit

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