Matrices listed below were spiked with certain level of NT-3 and the recovery rates were calculated by comparing the measured value to the expected amount of NT-3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-105
99
EDTA plasma(n=5)
88-104
95
heparin plasma(n=5)
87-95
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
NT-3 ELISA Kit allows for the in vitro quantitative determination of NT-3 concentrations in serum, plasma, tissue homogenates and other biological fluids.