Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Npc1 and the recovery rates were calculated by comparing the measured value to the expected amount of Npc1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
97
EDTA plasma(n=5)
88-101
93
heparin plasma(n=5)
85-103
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Npc1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-100%
86-96%
87-105%
EDTA plasma(n=5)
88-100%
82-100%
85-96%
heparin plasma(n=5)
81-99%
80-95%
83-96%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
O35604
Applications
ELISA
Applications Notes
Npc1 ELISA Kit allows for the in vitro quantitative determination of Npc1 concentrations in serum, plasma, tissue homogenates and other biological fluids.