Mouse Noggin - NOG ELISA Kit

Mfr.No.

E0954Mo

Sensitivity

9.57ng/L

Detection Range

20-4500ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

NOG;Noggin

Molecular Weight

25770

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0954Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1009.5	48.75	4.8
Intra-Assay	2	18	1404.5	47.71	3.4
Intra-Assay	3	18	385.6	26.9	7

Background

Essential for cartilage morphogenesis and joint formation. Inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patteing of the neural tube and somite (PubMed: 9585504, PubMed: 9603738). Inhibits chondrocyte differentiation through its interaction with GDF5 and, probably, GDF6 (By similarity).

Protocol

These standard curves of E0954Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.043	2.218	2.177
	2.394
1200ng/L	1.322	1.3	1.259
	1.279
600ng/L	0.733	0.765	0.724
	0.797
300ng/L	0.493	0.438	0.397
	0.383
150ng/L	0.234	0.224	0.183
	0.214
0ng/L	0.042	0.041	0
	0.04

Gene ID	18121
UniProt ID	P97466

Research Area	Developmental Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12108825	E0954Mo	48T	$320		Add to Cart
BHE12108825	E0954Mo	96T	$458		Add to Cart

Mouse Noggin - NOG ELISA Kit

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