Matrices listed below were spiked with certain level of NKB and the recovery rates were calculated by comparing the measured value to the expected amount of NKB in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-99
92
EDTA plasma(n=5)
85-103
92
heparin plasma(n=5)
87-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NKB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-104%
87-101%
89-103%
EDTA plasma(n=5)
85-97%
85-98%
85-100%
heparin plasma(n=5)
81-97%
81-99%
84-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P55099
Applications
ELISA
Applications Notes
NKB ELISA Kit allows for the in vitro quantitative determination of NKB concentrations in serum, plasma, tissue homogenates and other biological fluids.