Mouse NF-kappa-B Inhibitor Alpha

Mfr.No.

E2305Mo

Sensitivity

9.93ng/L

Detection Range

20-4500ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

IkappaBalpha;I-kappa-B-alpha;IkB-alpha;NF-kappa-B inhibitor alpha;NFKBIA

Molecular Weight

35071

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2305Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1680.5	46.96	2.8
Intra-Assay	2	18	438.8	23.67	5.4
Intra-Assay	3	18	1160.7	55.54	4.8

Background

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Protocol

These standard curves of E2305Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.149	2.019	1.989
	1.889
1200ng/L	0.947	1.019	0.988
	1.09
600ng/L	0.49	0.537	0.507
	0.584
300ng/L	0.187	0.239	0.209
	0.291
150ng/L	0.109	0.132	0.102
	0.156
0ng/L	0.032	0.03	0
	0.028

Gene ID	18035
UniProt ID	Q9Z1E3

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12110116	E2305Mo	48T	$320		Add to Cart
BHE12110116	E2305Mo	96T	$458		Add to Cart

Mouse NF-kappa-B Inhibitor Alpha - NFKBIA ELISA Kit

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