Mouse neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit

Mouse neutrophil gelatinase-associated lipocalin,NGAL ELISA Kit

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Mfr.No.

CSB-E09410m

Sensitivity

3.12 pg/mL

Detection Range

12.5 pg/mL-800 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

lipocalin 2, 24p3, MSFI, NGAL, OTTHUMP00000022240, migration-stimulating factor inhibitor, neutrophil gelatinase-associated lipocalin, oncogene 24p3, siderocalin

Recovery

The recovery of fish LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	97	93-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	91
	Range %	86-98
1:40	Average %	97
	Range %	91-102
1:80	Average %	84
	Range %	80-87
1:160	Average %	90
	Range %	83-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.082	0.086	0.084
500	0.190	0.200	0.195
250	0.483	0.444	0.464
125	0.945	0.959	0.952
62.5	1.672	1.555	1.614
0	2.591	2.670	2.631

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P11672

Applications	ELISA
Research Area	LCN2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506355	CSB-E09410m	96T	$695	Add to Cart
BHE10506355	CSB-E09410m	5x96T	$3336	Add to Cart
BHE10506355	CSB-E09410m	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.