Mouse Neutrophil Elastase

Mfr.No.

E0025Mo

Description

This sandwich kit is for the accurate quantitative detection of Mouse Neutrophil Elastase (also known as NE) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ELANE;Elastase-2;Leukocyte elastase;Neutrophil elastase

Reactivity

Mouse

Detection Range

0.1-40ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

28648

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.052ng/mL

Background

Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (By similarity). Capable of killing E.coli; probably digests outer membrane protein A (ompA) in E.coli (PubMed: 10947984).

Gene ID	50701
UniProt ID	Q3UP87
Antigen	ELANE

Research Area

Signal Transduction

Protocol

These standard curves of E0025Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/mL	2.042	2.043	2.013
	2.044
12ng/mL	1.367	1.337	1.307
	1.307
6ng/mL	0.762	0.696	0.666
	0.63
3ng/mL	0.307	0.354	0.324
	0.401
1.5ng/mL	0.198	0.18	0.15
	0.162
0ng/mL	0.022	0.03	0
	0.038

Precision

We measured random samples of E0025Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	10.3	0.44	4.3
Intra-Assay	2	18	16.5	0.78	4.7
Intra-Assay	3	18	3.5	0.16	4.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107953	E0025Mo	48T	$320		Add to Cart
BHE12107953	E0025Mo	96T	$458		Add to Cart

Mouse Neutrophil Elastase - NE ELISA Kit

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