Mouse neutrophil elastase,NE ELISA Kit

Mouse neutrophil elastase,NE ELISA Kit

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Mfr.No.

CSB-E04804m

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

elastase 2, neutrophil, ELA2, GE, HLE, HNE, NE, PMN-E, SCN1, bone marrow serine protease, elastase 2, neutrophil, granulocyte-derived elastase, leukocyte elastase, medullasin, neutrophil elastase, polymorphonuclear elastase

Recovery

The recovery of human NEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	90-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	97
	Range %	90-104
1:4	Average %	103
	Range %	97-109
1:8	Average %	89
	Range %	85-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.388	2.580	2.484	2.340
2500	1.836	1.947	1.892	1.748
1250	1.471	1.425	1.448	1.304
625	0.925	1.005	0.965	0.821
312.5	0.605	0.654	0.630	0.486
156	0.388	0.389	0.389	0.245
78	0.249	0.244	0.247	0.103
0	0.138	0.149	0.144

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q3UP87

Applications	ELISA
Research Area	ELA2

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.