Mouse Neurokinin -1 Receptor

Mfr.No.

E2020Mo

Sensitivity

1.51ng/L

Detection Range

2-700ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of MouseNeurokinin-1 Receptor (also known as NK1R) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

NK-1 receptor;NK-1R;SPR;Substance-P receptor;Tachykinin receptor 1;TACR 1;TACR1

Molecular Weight

46322

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2020Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	262.7	7.19	2.7
Intra-Assay	2	18	144.2	6.61	4.6
Intra-Assay	3	18	57.7	3.14	5.4
Intra-Assay	1	18	55.05	3.38	6.14
Intra-Assay	2	18	82.95	4.09	4.93
Intra-Assay	3	18	229.75	10.89	4.74

Background

This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P ; substance K ; neuromedin K.

Protocol

These standard curves of E2020Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
400ng/L	2.394	2.385	2.35
	2.376
200ng/L	1.301	1.296	1.261
	1.29
100ng/L	0.769	0.704	0.669
	0.639
50ng/L	0.4	0.419	0.385
	0.439
25ng/L	0.177	0.163	0.128
	0.149
0ng/L	0.034	0.035	0
	0.036

Gene ID	21336
UniProt ID	P30548

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109831	E2020Mo	48T	$320		Add to Cart
BHE12109831	E2020Mo	96T	$458		Add to Cart

Mouse Neurokinin -1 Receptor - NK1R ELISA Kit

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