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Home / ELISA Kit

Mouse Neural cell adhesion molecule L1 (L1CAM) ELISA Kit

SKU : BHE13706359

L1, also known as L1CAM, is a transmembrane protein; it is a neuronal cell adhesion molecule, member of the L1 protein family, of 200-220 kDa, and involved in axon guidance and cell migration with a strong implication in treatment-resistant cancers. L1CAM has also been designated CD171 . The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin superfamily of proteins. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration, and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH.

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Mfr.No.	KTE71200
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Neural cell adhesion molecule L1 (L1CAM) ELISA Kit employs a two-site sandwich ELISA to quantitate L1CAM.
Alternative Names	L1CAM; CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAML1; S10; SPG1; OTTHUMP00000025992; OTTHUMP00000063091; antigen identified by monoclonal antibody R1; neural cell adhesion molecule L1
Detection Method	Colorimetric
Kit Components	Mouse Neural cell adhesion molecule L1 microplate Mouse Neural cell adhesion molecule L1 standard Mouse Neural cell adhesion molecule L1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Neural cell adhesion molecule L1 (L1CAM) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse L1CAM. No significant cross-reactivity or interference between Mouse L1CAM and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	L1, also known as L1CAM, is a transmembrane protein; it is a neuronal cell adhesion molecule, member of the L1 protein family, of 200-220 kDa, and involved in axon guidance and cell migration with a strong implication in treatment-resistant cancers. L1CAM has also been designated CD171 . The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin superfamily of proteins. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration, and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH.
Notes	This Mouse Neural cell adhesion molecule L1 (L1CAM) ELISA Kit employs a two-site sandwich ELISA to quantitate L1CAM in samples. An antibody specific for L1CAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyL1CAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for L1CAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of L1CAM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Mouse Neural cell adhesion molecule L1 (L1CAM) ELISA Kit employs a two-site sandwich ELISA to quantitate L1CAM in samples. An antibody specific for L1CAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyL1CAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for L1CAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of L1CAM bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13706359	KTE71200	48 T	$339		Add to Cart
BHE13706359	KTE71200	96 T	$589		Add to Cart
BHE13706359	KTE71200	96 Tx5	$2629		Add to Cart
BHE13706359	KTE71200	96 Tx50	$24569		Add to Cart

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