Mouse Nerve growth factor,NGF ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04684m

Detection Range

62.5 pg/ml-4000 pg/ml

Sensitivity

15.6 pg/ml

Reactivity

Mouse

Sample Type

Serum, Tissue homogenates, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Recovery

The recovery of human RORC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	97-107
EDTA plasma (n=4)	92	89-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human RORC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	98
	Range %	93-103
1:4	Average %	84
	Range %	81-87
1:8	Average %	88
	Range %	83-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

UniProt ID	P01139
Antigen	nerve growth factor (beta polypeptide)

Applications

ELISA

Research Area

NGF

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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