Mouse Nepmucin/CD300g ELISA Kit PicoKine®

Mfr.No.

EK2207

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.027	0.053	0.101	0.207	0.423	0.868	1.375	1.962

Description

Mouse Nepmucin/CD300g Kit PicoKine® (96 Tests). Quantitate Mouse Cd300lg in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L19-I284

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Cd300lg

Sample Type

Cell culture supeatants, Serum, Plasma

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	354	1841	6397	340	1906	6655
Standard deviation	12.39	81	249.48	15.3	80.05	286.17
CV(%)	3.5%	4.4%	3.9%	4.5%	4.2%	4.3%

Background

Enables IgM binding activity. Acts upstream of or within immunoglobulin transcytosis in epithelial cells. Located in apical plasma membrane and basolateral plasma membrane. Orthologous to human CD300LG (CD300 molecule like family member g).

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	352	365	363	371	363	19.95	5.5%
Sample 2	1277	1265	1302	1287	1283	75.68	5.9%
Sample 3	4565	4542	4615	4787	4627	286.89	6.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	52685
Gene Name	Cd300lg
UniProt ID	Q1ERP8

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001753	EK2207	96 wells/kit	$750		Add to Cart

Mouse Nepmucin/CD300g ELISA Kit PicoKine®

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