Mouse NACHT, LRR and PYD domains-containing protein 3(NLRP3) ELISA kit

Mouse NACHT, LRR and PYD domains-containing protein 3(NLRP3) ELISA kit

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Mfr.No.

CSB-EL015871MO

Alternative Names

NLR family, pyrin domain containing 3, AGTAVPRL, AII, AII/AVP, AVP, C1orf7, CIAS1, CLR1.1, FCAS, FCU, FLJ95925, MWS, NALP3, PYPAF1, AII/AVP receptor-like, NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3, NACHT, LRR and PY

Reactivity

Mouse

Detection Range

6.25 pg/mL-400 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of dog IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-105
EDTA plasma (n=4)	92	85-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-97
1:2	Average %	89
	Range %	85-99
1:4	Average %	98
	Range %	92-105
1:8	Average %	93
	Range %	86-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.912	2.986	2.949	2.853
200	2.546	2.531	2.539	2.443
100	1.780	1.831	1.806	1.710
50	1.127	1.207	1.167	1.071
25	0.546	0.588	0.567	0.471
12.5	0.259	0.276	0.268	0.172
6.25	0.168	0.176	0.172	0.076
0	0.095	0.096	0.096	?

Species

Mus musculus

Sensitivity

1.56 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q8R4B8
Antigen	NLR family, pyrin domain containing 3

Applications

ELISA

Research Area

NLRP3

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10506303	CSB-EL015871MO	96T	$695	Add to Cart
BHE10506303	CSB-EL015871MO	5x96T	$3336	Add to Cart
BHE10506303	CSB-EL015871MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.