Mouse N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®

Mfr.No.

EK1155

Sensitivity

<20 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Mouse

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.081	0.181	0.249	0.456	0.675	1.084	1.564	2.030

Description

Mouse N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cdh2 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cadherin-2;Neural cadherin;N-cadherin;CD325;Cdh2;

Molecular Weight

99796 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D160-A724

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Cadherin-2

Subcellular Localization

Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1737	5669	22081	1767	5460	20442
Standard deviation	74.69	340.14	1236.53	102.48	425.88	1512.7
CV (%)	4.3%	6%	5.6%	5.8%	7.8%	7.4%

Protein Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence (PubMed:24952463). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. .

Background

Cadherin-2 (CDH2), also known as neural cadherin (NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1737	1604	1707	1747	1698	56.64	3.3%
Sample 2	5669	6007	5146	5724	5636	310.87	5.5%
Sample 3	22081	20274	22811	21203	21592	950.46	4.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	12558
Gene Name	CDH2
UniProt ID	P15116
Protein Name	Cadherin-2

Applications	ELISA
Research Area	Cadherins,Cell Adhesion,Cytoskeleton/ECM,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000883	EK1155	96 wells/kit	$499		Add to Cart

Mouse N-Cadherin-2 CDH2 CD325 ELISA Kit PicoKine®

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