Mouse Myostatin/GDF8 ELISA Kit PicoKine®

Mfr.No.

EK1266

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.076	0.150	0.185	0.292	0.520	0.839	1.496	2.092

Description

Mouse Myostatin/GDF8 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse MSTN in cell culture supeatants, tissue, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Growth/differentiation factor 8; GDF-8; Myostatin; Mstn; Gdf8

Immunogen

Expression system for standard: NS0; Immunogen sequence: D268-S376

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse Myostatin/GDF8

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Tissue lysate, Serum, Plasma

Assay Procedure Summary

Activate the sample

Cell culture supeatants: add activating reagent pro rata, i.e. add 50?l of Solution A into 100?l of sample, 10 min later, add 50?l of Solution B. PH 7.0-7.6.

Serum, plasma(heparin, EDTA): add activating reagent pro rata, i.e. add 20?l of Solution A into 40?l of sample, 10 min later, add 20?l of Solution B. PH 7.0-7.6.

It is unnecessary to activate the recombinant Myostatin/GDF8.

Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Anticoagulant

heparin or EDTA

Activating Reagent

Myostatin/GDF8 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant Myostatin/GDF8.
Solution A: 1N HCI: add 8.33ml of 12N HCI into 91.67ml of H₂O.
Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H₂O, add H₂O to adjust volume to 100ml.

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	51	227	971	59	238	984
Standard deviation	2.04	11.12	54.38	4.01	12.38	58.06
CV (%)	4.0%	4.9%	5.6%	6.8%	5.2%	5.9%

Protein Function

Acts specifically as a negative regulator of skeletal muscle growth.

Background

Myostatin (also known as growth differentiation factor 8, abbreviated GDF-8) is a myokine, a protein produced and released by myocytes that acts on muscle cells' autocrine function to inhibit myogenesis: muscle cell growth and differentiation. In humans it is encoded by the MSTN gene. It is mapped to 1 C1.1; 1 26.99 cM. Myostatin is a secreted growth differentiation factor that is a member of the TGF beta protein family. This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	51	55	46	51	50	3.19	6.3%
Sample 2	227	216	255	226	231	14.50	6.2%
Sample 3	971	973	959	989	973	10.67	1.0%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	17700
Gene Name	MSTN
UniProt ID	O08689
Tissue Specificity	Expressed specifically in developing and adult skeletal muscle. Weak expression in adipose tissue.

Applications	ELISA
Research Area	Neuroscience,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001500	EK1266	96 wells/kit	$499		Add to Cart

Mouse Myostatin/GDF8 ELISA Kit PicoKine®

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